Team:Warsaw/Calendar-Main/31 July 2008

From 2008.igem.org

(Difference between revisions)
Line 12: Line 12:
Elongation time: 30s <br>
Elongation time: 30s <br>
-
- optimalization of annealing temperature (gradient from 55&deg;C to 75&deg;C).
+
- optimalization of annealing temperature (gradient from 55&deg;C to 75&deg;C)<br>
- opimalization of number of cycles(15, 20, 25, 30, 35)
- opimalization of number of cycles(15, 20, 25, 30, 35)

Revision as of 15:11, 1 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 



1. Optimalization of PCR to obtain trunced A protein DNA fragment
Primers: AL+SacI AP+NotI

Elongation time: 30s

- optimalization of annealing temperature (gradient from 55°C to 75°C)
- opimalization of number of cycles(15, 20, 25, 30, 35)