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Team:Warsaw/Calendar-Main/16 May 2008
From 2008.igem.org
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Michał K.: | Michał K.: | ||
| - | 1. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - AID for | + | 1. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - AID for translational fusion. <br> |
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| - | 2. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA- | + | 2. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA-polymerase for translational fusion.<br> |
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| - | 3. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA- | + | 3. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA-polymerase for transcription fusion.<br> |
Primers: | Primers: | ||
Revision as of 15:34, 1 October 2008
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Michał K.: 1. PCR - AID for translational fusion. Elongation time: 60 sec 20 cycles
Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Elongation time: 4 minutes 20 cycles 3. PCR - T7 RNA-polymerase for transcription fusion. Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Elongation time: 4 minutes 20 cycles
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