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Team:Warsaw/Calendar-Main/14 May 2008
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Revision as of 15:55, 1 October 2008
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Michał K.: 1.Setup of 9 separate cultures from 9 colonies of transformants pMPMT5+AID (liquid LB+tetracycline). Elongation time: 60 sec 20 cycles 3. Optimization of conditions for PCR - T7 RNA polymerase for translation fusion; annealing temperature gradient (from 62°C to 82°C). Template DNA: E. coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> genomic DNA Elongation time: 4 minutes
20 cycles 4. Optimization of conditions for PCR - T7 RNA polymerase for transcription fusion; annealing temperature gradient (from 62°C to 82°C). Template DNA: E. coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> (negative control) genomic DNA
Elongation time: 4 minutes 20 cycles 5. Gel electrophoresis of PCR products. </p>
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