Team:Warsaw/Calendar-Main/31 July 2008
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5. Clean-up of digestion reaction. <br> | 5. Clean-up of digestion reaction. <br> | ||
6. Gel electrophoresis for estimation of DNA concentration. <br> | 6. Gel electrophoresis for estimation of DNA concentration. <br> | ||
- | + | 7. Overnight ligation: pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA. | |
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{{WarNotebookEnd}} | {{WarNotebookEnd}} |
Revision as of 15:55, 1 October 2008
1. Optimalization of PCR to obtain trunced A protein DNA fragment Elongation time: 30s Annealing temperature: 60°C 20 cycles 3. Gel electrophoresis and isolation of 250 bp band.
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