Michał K.:

1. PCR - AID for translational fusion.
Primers: AIDlNrH AIDpLinB
Template DNA: pTrc99A-AID
Annealing temperature: 55 °C

Elongation time: 60 sec

20 cycles

2. PCR - T7 RNA-polymerase for translational fusion.
Primers: T7lLinkB T7pXbSal

Template DNA: E. coli Rosetta genomic DNA

Annealing temperature: 62 °C

Elongation time: 4 minutes

20 cycles

3. PCR - T7 RNA-polymerase for transcription fusion.
Primers: T7lRBSHi T7pXbSal

Template DNA: E. coli Rosetta genomic DNA

Annealing temperature: 62 °C

Elongation time: 4 minutes

20 cycles

Michał L., Ewa, Marcin:

Plating colonies from the previous day on rifampicin