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Team:Warsaw/Calendar-Main/12 May 2008
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<p>Michał K.:</p> | <p>Michał K.:</p> | ||
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| + | <li> Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digestion</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a> with HindIII and NcoI (1x Tango buffer).</li> | ||
| - | < | + | <li> DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation of DNA</a> encoding AID from proper band (600 bp).</li> |
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| + | <li> Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digestion</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> with HindIII and NcoI (1x Tango buffer), <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation</a> (CIAP).</li> | ||
| + | <li> DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> vector from proper band (4250 bp).</li> | ||
| + | <li>Electrophoresis of isolated DNA</li> | ||
| + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments. </li> | ||
| + | </ol></p> | ||
<html><div align="center"><img src="http://openwetware.org/images/e/e1/UW_AID.jpg" height="340"/></div></html> | <html><div align="center"><img src="http://openwetware.org/images/e/e1/UW_AID.jpg" height="340"/></div></html> | ||
{{WarNotebookEnd}} | {{WarNotebookEnd}} | ||
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Michał K.:
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