Team:Warsaw/Calendar-Main/15 May 2008

From 2008.igem.org

(Difference between revisions)
Line 11: Line 11:
<p>3. Gel electrophoresis - choice of proper clones (all checked colonies).</p>
<p>3. Gel electrophoresis - choice of proper clones (all checked colonies).</p>
-
<p>Michał L., Ewa, Marcin.</p>
 
-
<p>Overnight inoculation and induction:</p>
 
-
<p>Top10 + 0,5 mM IPTG</p>
 
-
<p>pMPMAID + Glc + 0,01% L-Ara</p>
 
-
<p>pTRCAID + Glc + 0,5 mM IPTG</p>
 
</html>
</html>
<!-- do not remove this! -->
<!-- do not remove this! -->
{{WarNotebookEnd}}
{{WarNotebookEnd}}

Revision as of 16:03, 1 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Michał K.

1. Isolation of hypothetical pMPM-T5+AID plasmids from cultures inoculated on previous day.

2. Restriction digestion of plasmids with HindIII and NcoI (1xTango buffer) - construct control.

3. Gel electrophoresis - choice of proper clones (all checked colonies).

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/15_May_2008"