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Team:Warsaw/Calendar-Main/19 May 2008
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<p>1. Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008">16 May</a> PCR's) and DNA <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> from proper bands (600 bp for AID lane and 2700 bp for T7 polymerase lanes).</html> | <p>1. Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008">16 May</a> PCR's) and DNA <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> from proper bands (600 bp for AID lane and 2700 bp for T7 polymerase lanes).</html> | ||
| + | <p>Michał L., Ewa, Marcin.</p> | ||
| + | <p>Overnight inoculation and induction:</p> | ||
| + | <p>Top10 + 0,5 mM IPTG</p> | ||
| + | <p>pMPMAID + Glc + 0,01% L-Ara</p> | ||
| + | <p>pTRCAID + Glc + 0,5 mM IPTG</p> | ||
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{{WarNotebookEnd}} | {{WarNotebookEnd}} | ||
Revision as of 16:04, 1 October 2008
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