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Team:Warsaw/Calendar-Main/20 May 2008
From 2008.igem.org
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<p>Michał K: <br> | <p>Michał K: <br> | ||
| - | 1.<html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase - | + | 1.<html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase - optimization (temperature gradient 60°C - 80°C).</p> |
<p>Primers: </p> | <p>Primers: </p> | ||
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35 cycles <br> | 35 cycles <br> | ||
| - | 2. | + | 2. Optimization of <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase - MgCl2 cocentration and number of cycles. |
<p>Primers: </p> | <p>Primers: </p> | ||
Revision as of 16:10, 1 October 2008
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Michał K: Primers:
AIDlNrH and
T7pXbSal Primers:
Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion Elongation temperature: 73°C 3. Gel electrophoresis of PCR products.
Michał L., Ewa, Marcin: Plating colonies from the previous day on rifampicin
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