"
Team:UNIPV-Pavia/Protocols/Resuspension
From 2008.igem.org
(Difference between revisions)
| Line 16: | Line 16: | ||
<h1>Plasmid resuspension from IGEM paper spots</h1> | <h1>Plasmid resuspension from IGEM paper spots</h1> | ||
| + | '''(estimated time: 25 min for every part if you use scalpel/tweezers; 35 min for every part if you use punch tool)''' | ||
'''Materials needed:''' | '''Materials needed:''' | ||
*'''Pre-warmed at 42°C TE''' | *'''Pre-warmed at 42°C TE''' | ||
| Line 29: | Line 30: | ||
*Put the cut paper into the 0.5 ml tube. | *Put the cut paper into the 0.5 ml tube. | ||
*Incubate at 42°C for 20 min; then vortex and spin down. | *Incubate at 42°C for 20 min; then vortex and spin down. | ||
| - | + | <br> | |
Revision as of 22:55, 9 June 2008
 Home
|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
Plasmid resuspension from IGEM paper spots
(estimated time: 25 min for every part if you use scalpel/tweezers; 35 min for every part if you use punch tool) Materials needed:
- Pre-warmed at 42°C TE
- Desired spot location information
- Scalpel and tweezers (or punch tool)
- diH20
- 99% ethanol
- 0.5 ml tubes
- Put 10 µl of pre-warmed TE into a 0.5 ml tube.
- Cut paper spots using scalpel and tweezers (or punch tool, following provided instructions); if you use scalpel and tweezers, try to cut pieces of about the same dimension of the punch tool.
- Clean scalpel and tweezers (or punch tool) with water and ethanol every time you cut a spot.
- Put the cut paper into the 0.5 ml tube.
- Incubate at 42°C for 20 min; then vortex and spin down.
