Team:Warsaw/Calendar-Main/15 May 2008

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<li> Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digestion</a> of plasmids with HindIII and NcoI (1xTango buffer) - construct control.</li>
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<li> Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of plasmids with HindIII and NcoI (1xTango buffer) - construct control.</li>

Revision as of 17:42, 1 October 2008

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Michał K.

  1. Isolation of hypothetical pMPM-T5+AID plasmids from cultures inoculated on previous day.
  2. Restriction digest of plasmids with HindIII and NcoI (1xTango buffer) - construct control.
  3. Gel electrophoresis - choice of proper clones (all checked colonies).

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