Team:Warsaw/Calendar-Main/28 July 2008

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<p>1. Digestion of pACYC177+OmpA_omega_deltaA_alpha (from 25 July) with BamHI and NotI. DNA ends blunting with Klenow fragment (3 hr). <br>
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<p>1. Digest of pACYC177+OmpA_omega_deltaA_alpha (from 25 July) with BamHI and NotI. DNA ends blunting with Klenow fragment (3 hr). <br>
2. Gel elctrophoresis and gel-out of proper band - 4300 bp. <br>
2. Gel elctrophoresis and gel-out of proper band - 4300 bp. <br>
3. Ligation of isolated DNA fragment (1 hr). <br>
3. Ligation of isolated DNA fragment (1 hr). <br>

Revision as of 18:02, 1 October 2008

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1. Digest of pACYC177+OmpA_omega_deltaA_alpha (from 25 July) with BamHI and NotI. DNA ends blunting with Klenow fragment (3 hr).
2. Gel elctrophoresis and gel-out of proper band - 4300 bp.
3. Ligation of isolated DNA fragment (1 hr).
4. Transformation of E. coli TOP10 strain with ligation.
5. Transformants plating on LB + kanamycin.



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