Team:Warsaw/Calendar-Main/22 July 2008

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2. Control digest of isolated plasmids with BamHI and SacI . </p>
2. Control digest of isolated plasmids with BamHI and SacI . </p>
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<p>Paweł: ligation transformed into TOP10 strain, plated on kanamycin plate</p>

Revision as of 18:16, 1 October 2008

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Cloning of protein A DNA to OmpA constructs
1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_omega).
2. Control digest of isolated plasmids with BamHI and SacI .

Paweł: ligation transformed into TOP10 strain, plated on kanamycin plate

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