Team:Warsaw/Calendar-Main/18 July 2008

From 2008.igem.org

(Difference between revisions)
Line 2: Line 2:
<!-- do not edit above me! -->
<!-- do not edit above me! -->
 +
<html>
 +
<h3>Cloning of protein A DNA to OmpA constructs</h3>
 +
<p><ol>
 +
<li>Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega and pACYC177+OmpA_A_alpha. Primers used:
-
<p>'''Cloning of protein A DNA to OmpA constructs'''<br>
 
-
1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega and pACYC177+OmpA_A_alpha. Primers used:
 
-
<html>
 
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a>
-
</html><br>
+
</li>
-
2. Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha plate) inoculated to liquid LB with kanamycin. <br>
+
<li> Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha plate) inoculated to liquid LB with kanamycin. </li></ol>
</p>
</p>
<p>Paweł: pET15b vector purified.
<p>Paweł: pET15b vector purified.
-
Digested of pET15b and pACYClac+OmpA+omega with BamHI and NdeI<br>Gel-out of OmpA+omega (cutted from pACYClac+OmpA+omega, ~1350 bp)</p>
+
Digest of pET15b and pACYClac+OmpA+omega with BamHI and NdeI<br>Gel-out of OmpA+omega (cutted from pACYClac+OmpA+omega, ~1350 bp)</p>
<p>Ligation of gel-outed fragment into digested pEt15b
<p>Ligation of gel-outed fragment into digested pEt15b
</p>
</p>
-
 
+
</html>
<!-- do not remove this! -->
<!-- do not remove this! -->
{{WarNotebookEnd}}
{{WarNotebookEnd}}

Revision as of 18:21, 1 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Cloning of protein A DNA to OmpA constructs

  1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega and pACYC177+OmpA_A_alpha. Primers used: AL+SacI and AP+NotI
  2. Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha plate) inoculated to liquid LB with kanamycin.

Paweł: pET15b vector purified. Digest of pET15b and pACYClac+OmpA+omega with BamHI and NdeI
Gel-out of OmpA+omega (cutted from pACYClac+OmpA+omega, ~1350 bp)

Ligation of gel-outed fragment into digested pEt15b

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/18_July_2008"