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Team:Warsaw/Calendar-Main/21 June 2008
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| + | <h3>Michał L., Ewa, Marcin</h3> | ||
| + | <h4>PCR</h4> | ||
| + | <table id="result"> | ||
| + | <tr><th>Product</th><th>Template</th><th>Primers</th><th>Product length</th></tr> | ||
| + | <tr><th>linker-A</th><td>pDRIVE-TapTag</td><td>AL+link10+homo2 + AP+NotI </td><td>470 bp</td></tr> | ||
| + | <tr><th>alpha-linker</th><td>pUC19</td><td>AlphaL+SacI + AlphaP+link10+homo2 </td><td>1022 bp</td></tr> | ||
| + | <tr><th>omega-linker</th><td>pUC19</td><td>OmegaL+SacI + OmegaP+link10+homo2 </td><td>779 bp</td></tr> | ||
| + | </table> | ||
| + | <h4>Universal PCR program for A, alpha and omega</h4> | ||
| + | <table id="result"> | ||
| + | <tr><th>Temperature</th><th>Time</th></tr> | ||
| + | <tr><td>94°C</td><td>4:00</td></tr> | ||
| + | <tr><td>94°C</td><td>0:30</td><td rowspan="3">28 cycles</td></tr> | ||
| + | <tr><td>50°C</td><td>0:45</td></tr> | ||
| + | <tr><td>72°C</td><td>2:00</td></tr> | ||
| + | <tr><td>72°C</td><td>10:00</td></tr> | ||
| + | <tr><td>4°C</td><td>infinite</td></tr> | ||
| + | </table> | ||
| - | + | <p>Polymerase: homemade Pfu-Turbo</p> | |
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Revision as of 18:25, 1 October 2008
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Michał L., Ewa, MarcinPCR
Universal PCR program for A, alpha and omega
Polymerase: homemade Pfu-Turbo
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