Team:Warsaw/Calendar-Main/10 June 2008

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<html><h3>Michał L., Ewa, Marcin</h3>
<html><h3>Michał L., Ewa, Marcin</h3>
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<p><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Plasmid isolation</a> from overnight culture of our transformants.<br>
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<ul><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Plasmid isolation</a> from overnight culture of our transformants.</li>
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Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.<br>
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<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.</li>
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Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.<br>
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<li>Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.</li>
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Control digest of pMPM-T5 + T7 RNA-polymerase with EcoRI.<br>
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<li>Control digest of pMPM-T5 + T7 RNA-polymerase with EcoRI.</li>
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Control digest of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.<br>
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<li>Control digest of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.</li>
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DNA gel electrophoresis of digestion products.<br>
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<li>DNA gel electrophoresis of digestion products.</li>
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Picking out proper colonies of transformants.<br>
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<li>Picking out proper colonies of transformants.</li>
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Michał L., Ewa, Marcin

  • Plasmid isolation from overnight culture of our transformants.
  • Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.
  • Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.
  • Control digest of pMPM-T5 + T7 RNA-polymerase with EcoRI.
  • Control digest of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.
  • DNA gel electrophoresis of digestion products.
  • Picking out proper colonies of transformants.