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Team:Warsaw/Calendar-Main/2 July 2008
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<h3>Preparation of constructs with OmpA protein fusions</h3> | <h3>Preparation of constructs with OmpA protein fusions</h3> | ||
<p><ol> | <p><ol> | ||
| - | <li> PCR on OmpA_linker and linker_alpha with | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on OmpA_linker and linker_alpha with |
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">AlphaP_XB</a> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">AlphaP_XB</a> | ||
primers (20 cycles, annealing length 1 min 15 s, annealing temperature 57°C)</li> | primers (20 cycles, annealing length 1 min 15 s, annealing temperature 57°C)</li> | ||
| - | <li> PCR on OmpA_linker and linker_omega with | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on OmpA_linker and linker_omega with |
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a> | ||
Revision as of 18:27, 1 October 2008
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Preparation of constructs with OmpA protein fusions
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