Team:Warsaw/Calendar-Main/2 July 2008

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Revision as of 18:33, 1 October 2008


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PCR on OmpA_linker and linker_alpha with OmpaL_N and AlphaP_XB primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57°C).
PCR on OmpA_linker and linker_omega with OmpaL_N and OmegaP_EPB primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57°C).
Gel electrophoresis of PCR products and gel-out of OmpA_alpha and OmpA_omega.
Overnight digest of purified OmpA_alpha and OmpA_omega DNA with NdeI and BamHI.
Overnight digest of pET15b plasmid with NdeI and BamHI, dephosphorylation with CIAP.

@@ Line 10: / Line 10: @@
 <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">AlphaP_XB</a>
-  primers (20 cycles, annealing length 1 min 15 s, annealing temperature 57&deg;C).</li>
+  primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57&deg;C).</li>
 <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on OmpA_linker and linker_omega with
 <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a>
-  primers (20 cycles, annealing length 1 min 15 s, annealing temperature 57&deg;C).</li>
+  primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57&deg;C).</li>
 <li> Gel electrophoresis of PCR products and
 <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of OmpA_alpha and OmpA_omega. </li>