Team:Warsaw/Calendar-Main/16 May 2008

From 2008.igem.org

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<h4>Michał K.:</h4>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - AID for translational fusion. <br>
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Michał K.:
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Primers:
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1. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - AID for translational fusion. <br>
 
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Primers:
 
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDpLinB">AIDpLinB</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDpLinB">AIDpLinB</a>
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Template DNA: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a>
Template DNA: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a>
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</html> <br>
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<br>
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Annealing temperature: 55 °C
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Elongation temperature: 55 °C<br>
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Elongation time: 60 sec
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Elongation time: 60 sec<br>
20 cycles
20 cycles
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</li>
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<li> <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA-polymerase for translational fusion.<br>
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2. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA-polymerase for translational fusion.<br>
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Primers:
Primers:
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<html>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7lLinkB">T7lLinkB</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7lLinkB">T7lLinkB</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a>
<br>
<br>
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</html>
 
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Template DNA: E. coli Rosetta genomic DNA
 
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Annealing temperature: 62 °C
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Template DNA: <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a>  genomic DNA<br>
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Elongation temperature: 62 °C<br>
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Elongation time: 4 minutes
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Elongation time: 4 minutes<br>
20 cycles<br>
20 cycles<br>
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<br>
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</li>
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3. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA-polymerase for transcription fusion.<br>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA-polymerase for transcription fusion.<br>
Primers:
Primers:
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<html>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7lRBSHi">T7lRBSHi</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7lRBSHi">T7lRBSHi</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a>
<br>
<br>
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</html>
 
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Template DNA: E. coli Rosetta genomic DNA
 
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Annealing temperature: 62 °C
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Template DNA: <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a>  genomic DNA<br>
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Elongation time: 4 minutes
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Elongation temperature: 62 °C<br>
-
 
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20 cycles
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 +
Elongation time: 4 minutes<br>
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20 cycles
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</li>
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</p>
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</html>
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Revision as of 18:35, 1 October 2008

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Michał K.:

  1. PCR - AID for translational fusion.
    Primers: AIDlNrH AIDpLinB
    Template DNA: pTrc99A-AID
    Elongation temperature: 55 °C
    Elongation time: 60 sec
    20 cycles
  2. PCR - T7 RNA-polymerase for translational fusion.
    Primers: T7lLinkB T7pXbSal
    Template DNA: E. coli Rosetta genomic DNA
    Elongation temperature: 62 °C
    Elongation time: 4 minutes
    20 cycles
  3. PCR - T7 RNA-polymerase for transcription fusion.
    Primers: T7lRBSHi T7pXbSal
    Template DNA: E. coli Rosetta genomic DNA
    Elongation temperature: 62 °C
    Elongation time: 4 minutes
    20 cycles


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