Team:Warsaw/Calendar-Main/18 July 2008

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<li> Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha plate) inoculated to liquid LB with kanamycin. </li></ol>
<li> Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha plate) inoculated to liquid LB with kanamycin. </li></ol>
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<h3>Paweł</h3>
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<p>Paweł: pET15b vector purified.
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<p><ol><li>Ligation transformed into TOP10 strain and plated on LB+kanamycin</li>
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Digest of pET15b and pACYClac+OmpA+omega with BamHI and NdeI<br>Gel-out of OmpA+omega (cutted from pACYClac+OmpA+omega, ~1350 bp)</p>
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<p>Ligation of gel-outed fragment into digested pEt15b
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Revision as of 18:52, 1 October 2008

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Cloning of protein A DNA to OmpA constructs

  1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega and pACYC177+OmpA_A_alpha. Primers used: AL+SacI and AP+NotI
  2. Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha plate) inoculated to liquid LB with kanamycin.

Paweł

  1. Ligation transformed into TOP10 strain and plated on LB+kanamycin

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