Team:Warsaw/Calendar-Main/29 May 2008

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Piotr: <br>
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<h4>Piotr:</h4>
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1. Add 50 μL E.coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB.
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<p><ol>
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<li> Added 50 μL <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a>  carrying pMPM-T5+AID (in liquid LB) into liquid LB.</li>
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2. Add 50 μL E.coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose.
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<li> Added 50 μL <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a>  carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose.</li>
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3. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB.
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<li> Added 50 μL <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a>  carrying pTrc99A+AID (in liquid LB) into liquid LB.</li>
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4. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB + 1mM IPTG.
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<li> Added 50 μL <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a>  carrying pTrc99A+AID (in liquid LB) into liquid LB + 1mM IPTG.</li>
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5. Wait for 5 hours.
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<li> Waiting 5 hours.</li>
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6. OD measurement.
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<li> OD measurement.</li>
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7. 1 ml (2x) from p.1, p.2, p.3, p.4 (LB + rifampicin).
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<li> 1 ml (2x) from p.1, p.2, p.3, p.4 (LB + rifampicin). </li>
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</ol></p>
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Revision as of 18:52, 1 October 2008

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Piotr:

  1. Added 50 μL E. coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> carrying pMPM-T5+AID (in liquid LB) into liquid LB.
  2. Added 50 μL E. coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose.
  3. Added 50 μL E. coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> carrying pTrc99A+AID (in liquid LB) into liquid LB.
  4. Added 50 μL E. coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> carrying pTrc99A+AID (in liquid LB) into liquid LB + 1mM IPTG.
  5. Waiting 5 hours.
  6. OD measurement.
  7. 1 ml (2x) from p.1, p.2, p.3, p.4 (LB + rifampicin).


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