Team:Warsaw/Calendar-Main/16 May 2008

From 2008.igem.org

(Difference between revisions)
Line 15: Line 15:
Template DNA: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a>
Template DNA: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a>
  <br>
  <br>
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Elongation temperature: 55 °C<br>
+
Annealing temperature: 55 °C<br>
Elongation time: 60 sec<br>
Elongation time: 60 sec<br>
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Template DNA: <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a>  genomic DNA<br>
Template DNA: <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a>  genomic DNA<br>
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Elongation temperature: 62 °C<br>
+
Annealing temperature: 62 °C<br>
Elongation time: 4 minutes<br>
Elongation time: 4 minutes<br>
Line 53: Line 53:
Template DNA: <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a>  genomic DNA<br>
Template DNA: <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a>  genomic DNA<br>
-
Elongation temperature: 62 °C<br>
+
Annealing temperature: 62 °C<br>
Elongation time: 4 minutes<br>
Elongation time: 4 minutes<br>

Revision as of 20:57, 1 October 2008

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Michał K.:

  1. PCR - AID for translational fusion.
    Primers: AIDlNrH AIDpLinB
    Template DNA: pTrc99A-AID
    Annealing temperature: 55 °C
    Elongation time: 60 sec
    20 cycles
  2. PCR - T7 RNA-polymerase for translational fusion.
    Primers: T7lLinkB T7pXbSal
    Template DNA: E. coli Rosetta genomic DNA
    Annealing temperature: 62 °C
    Elongation time: 4 minutes
    20 cycles
  3. PCR - T7 RNA-polymerase for transcription fusion.
    Primers: T7lRBSHi T7pXbSal
    Template DNA: E. coli Rosetta genomic DNA
    Annealing temperature: 62 °C
    Elongation time: 4 minutes
    20 cycles