Team:Warsaw/Calendar-Main/15 May 2008

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<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5+AID plasmids from cultures inoculated on previous day.</li>
<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5+AID plasmids from cultures inoculated on previous day.</li>

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Michał K.:

  1. Isolation of hypothetical pMPM-T5+AID plasmids from cultures inoculated on previous day.
  2. Restriction digest of plasmids with HindIII and NcoI (1xTango buffer) - construct control.
  3. Gel electrophoresis - choice of proper clones (all checked colonies).

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