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Team:Warsaw/Calendar-Main/19 May 2008
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| - | + | <h4>Piotr:</h4> | |
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<p>1. Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008">16 May</a> PCR's) and DNA <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> from proper bands (600 bp for AID lane and 2700 bp for T7 polymerase lanes).</html> | <p>1. Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008">16 May</a> PCR's) and DNA <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> from proper bands (600 bp for AID lane and 2700 bp for T7 polymerase lanes).</html> | ||
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| + | <h4>Michał L., Ewa, Marcin:</h4> | ||
<p>Overnight inoculation and induction:</p> | <p>Overnight inoculation and induction:</p> | ||
<p>Top10 + 0,5 mM IPTG</p> | <p>Top10 + 0,5 mM IPTG</p> | ||
| - | <p> | + | <p>pMPM-AID + Glc + 0,01% L-Ara</p> |
| - | <p> | + | <p>pTrc99a-AID + Glc + 0,5 mM IPTG</p> |
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Revision as of 21:16, 1 October 2008
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Piotr:1. Gel electrophoresis (16 May PCR's) and DNA isolation from proper bands (600 bp for AID lane and 2700 bp for T7 polymerase lanes).
Michał L., Ewa, Marcin:Overnight inoculation and induction: Top10 + 0,5 mM IPTG pMPM-AID + Glc + 0,01% L-Ara pTrc99a-AID + Glc + 0,5 mM IPTG {{WarNotebookEnd}} |
