Team:Warsaw/Calendar-Main/19 May 2008

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(Difference between revisions)
Line 9: Line 9:
<h4>Michał L., Ewa, Marcin:</h4>
<h4>Michał L., Ewa, Marcin:</h4>
-
<p>Overnight inoculation and induction:</p>  
+
<p>1. Overnight inoculation and induction:</p>  
<p>Top10 + 0,5 mM IPTG</p>
<p>Top10 + 0,5 mM IPTG</p>
<p>pMPM-AID + Glc + 0,01% L-Ara</p>
<p>pMPM-AID + Glc + 0,01% L-Ara</p>

Revision as of 21:17, 1 October 2008

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Piotr:

1. Gel electrophoresis (16 May PCR's) and DNA isolation from proper bands (600 bp for AID lane and 2700 bp for T7 polymerase lanes).

Michał L., Ewa, Marcin:

1. Overnight inoculation and induction:

Top10 + 0,5 mM IPTG

pMPM-AID + Glc + 0,01% L-Ara

pTrc99a-AID + Glc + 0,5 mM IPTG

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