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Team:Warsaw/Calendar-Main/15 July 2008
From 2008.igem.org
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<h3>Cloning of protein Z DNA to OmpA constructs<br> | <h3>Cloning of protein Z DNA to OmpA constructs<br> | ||
Michał K.</h3> | Michał K.</h3> | ||
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<h3>Cloning of protein A DNA to geneart plasmid in place of protein Z DNA<br>Piotr</h3> | <h3>Cloning of protein A DNA to geneart plasmid in place of protein Z DNA<br>Piotr</h3> | ||
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| + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from bacterial cultures inoculated on previous day (pKS+A and pGeneart+Z). </li> | ||
| + | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of pKS+A and pGeneart+Z with SacI and NotI, <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing"> pGeneart dephosphorylation with CIAP</a>.</li> | ||
| + | <li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands </li> | ||
| + | <li> </li></ol></p> | ||
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Revision as of 21:18, 1 October 2008
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Cloning of protein Z DNA to OmpA constructs
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