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Team:Warsaw/Calendar-Main/20 May 2008
From 2008.igem.org
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| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase - optimization (temperature gradient 60°C - 80°C).</p> | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase - optimization (annealing temperature gradient 60°C - 80°C).</p> |
<p>Primers: </p> | <p>Primers: </p> | ||
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Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion <br> | Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion <br> | ||
| - | + | Annealing temperature: 73°C<br> | |
Elongation time: 4 minutes <br> | Elongation time: 4 minutes <br> | ||
</li> | </li> | ||
Revision as of 21:18, 1 October 2008
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Michał K:
Michał L., Ewa, Marcin:Plating colonies from the previous day on rifampicin
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