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Team:Warsaw/Calendar-Main/15 July 2008
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<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of pKS+A and pGeneart+Z with SacI and NotI, <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing"> pGeneart dephosphorylation with CIAP</a>.</li> | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of pKS+A and pGeneart+Z with SacI and NotI, <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing"> pGeneart dephosphorylation with CIAP</a>.</li> | ||
<li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands </li> | <li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands </li> | ||
| - | <li> </li></ol></p> | + | <li> Overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of pGeneart and A </li> |
| + | </ol></p> | ||
</html> | </html> | ||
Revision as of 21:20, 1 October 2008
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Cloning of protein Z DNA to OmpA constructs
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