Team:Warsaw/Calendar-Main/18 July 2008

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<h3>Cloning of protein A DNA to pET15b+Omp-alfa plasmid<br>Antoni</h3>
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Revision as of 21:47, 1 October 2008

Gallery Bricks Notebook Team Project Home


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Cloning of protein A DNA to OmpA constructs

  1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega and pACYC177+OmpA_A_alpha. Primers used: AL+SacI and AP+NotI
  2. Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha plate) inoculated to liquid LB with kanamycin.

Paweł

  1. Ligation transformed into TOP10 strain and plated on LB+kanamycin


Cloning of protein A DNA to pET15b+Omp-alfa plasmid
Antoni

  1. Isolation of plasmids from cultures inocluated on previous day (pGeneart+A).


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