Team:Warsaw/Calendar-Main/8 July 2008

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<h4>Michał K.</h4>
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<h3>Preparation of constructs with OmpA protein fusions</h3>
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<h3>Preparation of constructs with OmpA protein fusions<br> Piotr</h3>
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<h3>Preparation of construct pKS with A protein</h3>
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<h3>Preparation of construct pKS with A protein<br>
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Michał L., Marcin:</h3>
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<li> Inactivation of digestion enzymes and CIAP</li>
<li> Inactivation of digestion enzymes and CIAP</li>

Revision as of 22:32, 1 October 2008

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Preparation of constructs with OmpA protein fusions
Piotr

  1. Transformation of E. coli TOP10 strain with ligation from previous day
  2. Transformants plating on LB + kanamycin


Preparation of construct pKS with A protein
Michał L., Marcin:

  1. Inactivation of digestion enzymes and CIAP
  2. Ligation of digested PCR product and pKS 2h at room temperature
  3. Transformation of E. coli TOP10 strain with 7 µl of ligation mix
  4. Transformants plating on LB + ampicillin + X-gal + IPTG

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