Team:Warsaw/Calendar-Main/24 June 2008

From 2008.igem.org

(Difference between revisions)
Line 24: Line 24:
<h3>PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct.<br> Lane 1 - 25 cycles, lane 2 - 20 cycles.</h3>
<h3>PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct.<br> Lane 1 - 25 cycles, lane 2 - 20 cycles.</h3>
-
https://static.igem.org/mediawiki/2008/0/08/Tranlacyjna_pcr_29_05_2008.jpg
+
<img src="https://static.igem.org/mediawiki/2008/0/08/Tranlacyjna_pcr_29_05_2008.jpg">
 +
 
-
</p>
 

Revision as of 23:13, 1 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Preparation of pMPMT5-AID+AIDT7 construct

PCR - translation fusion: AID + T7 RNA-polimerase
Primers: AIDlNrH and T7pXbSal
Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion
Elongation temperature: 55°C
Elongation time: 4 minutes
Number of cycles: 20 and 25 (2 repeats)

Gel electrophoresis and DNA isolation from proper band (obtained with 20 cycles)

PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct.
Lane 1 - 25 cycles, lane 2 - 20 cycles.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/24_June_2008"