Team:Beijing Normal/Notebook

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**27th July          Have a discussion with Tsinghua team about the transformation efficiency.  
**27th July          Have a discussion with Tsinghua team about the transformation efficiency.  
We generalize some key words from the discussion:  
We generalize some key words from the discussion:  
 +
1. Make sure the concentration of the plasmids from the biobrick is enough for transformation.  
1. Make sure the concentration of the plasmids from the biobrick is enough for transformation.  
 +
2. Keep a low temprature when enzyme digestion is performed  
2. Keep a low temprature when enzyme digestion is performed  
 +
3. The transformation product are always incubated for more than 1h.  
3. The transformation product are always incubated for more than 1h.  
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Retrieved from "https://2008.igem.org/Beijing_Normal/27_July_2008"
 
**9th September      We and Tsinghua team get together in our lab. We have a happy time together and share a lot.  
**9th September      We and Tsinghua team get together in our lab. We have a happy time together and share a lot.  
 +
 +
**10th September    We provide Tsinghua Team with the cells harboring pSB1AC3 plasmids. Because they found their transformation effeciency is relatively low.
 +
 +
**17th September    Tsinghua Team told us that the vector that we provided proved to work well. We are all pretty happy about this positive result[[User:Sally730|Sally730]]

Revision as of 08:21, 2 October 2008

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Bnu.jpg

Dynamics

  • == Communications ==
    • 17th June Attend the Asian Workshop meeting.
    • 17th July Meet up with Tsinghua Team, and learn fron their experiences.
    • 25th July With Invitrogen.Co. Ltd and Tsinghua team to sign up a contract.
    • 27th July Have a discussion with Tsinghua team about the transformation efficiency.

We generalize some key words from the discussion:

1. Make sure the concentration of the plasmids from the biobrick is enough for transformation.

2. Keep a low temprature when enzyme digestion is performed

3. The transformation product are always incubated for more than 1h.


    • 9th September We and Tsinghua team get together in our lab. We have a happy time together and share a lot.
    • 10th September We provide Tsinghua Team with the cells harboring pSB1AC3 plasmids. Because they found their transformation effeciency is relatively low.
    • 17th September Tsinghua Team told us that the vector that we provided proved to work well. We are all pretty happy about this positive resultSally730


July
MTWTFSS
  [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/1_July_2008&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/2_July_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/3_July_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/4_July_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/5_July_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/6_July_2008&action=edit 6]
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/7_July_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/8_July_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/9_July_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/10_July_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/11_July_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/12_July_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/13_July_2008&action=edit 13]
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/14_July_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/15_July_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/16_July_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/17_July_2008&action=edit 17] [http://2008.igem.org/Beijing_Normal/18_July_2008 18] [http://2008.igem.org/Beijing_Normal/19_July_2008 19] [http://2008.igem.org/Beijing_Normal/20_July_2008 20]
[http://2008.igem.org/Beijing_Normal/21_July_2008 21] [http://2008.igem.org/Beijing_Normal/22_July_2008 22] [http://2008.igem.org/Beijing_Normal/23_July_2008 23] [http://2008.igem.org/Beijing_Normal/24_July_2008 24] [http://2008.igem.org/Beijing_Normal/25_July_2008 25] [http://2008.igem.org/Beijing_Normal/26_July_2008 26] [http://2008.igem.org/Beijing_Normal/27_July_2008 27]
[http://2008.igem.org/Beijing_Normal/28_July_2008 28] [http://2008.igem.org/Beijing_Normal/29_July_2008 29] [http://2008.igem.org/Beijing_Normal/30_July_2008 30] [http://2008.igem.org/Beijing_Normal/31_July_2008 31]
August
MTWTFSS
        [http://2008.igem.org/Beijing_Normal/1_August_2008 1] [http://2008.igem.org/Beijing_Normal/2_August_2008 2] [http://2008.igem.org/Beijing_Normal/3_August_2008 3]
[http://2008.igem.org/Beijing_Normal/4_August_2008 4] [http://2008.igem.org/Beijing_Normal/5_August_2008 5] [http://2008.igem.org/Beijing_Normal/6_August_2008 6] [http://2008.igem.org/Beijing_Normal/7_August_2008 7] [http://2008.igem.org/Beijing_Normal/8_August_2008 8] [http://2008.igem.org/Beijing_Normal/9_August_2008 9] [http://2008.igem.org/Beijing_Normal/10_August_2008 10]
[http://2008.igem.org/Beijing_Normal/11_August_2008 11] [http://2008.igem.org/Beijing_Normal/12_August_2008 12] [http://2008.igem.org/Beijing_Normal/13_August_2008 13] [http://2008.igem.org/Beijing_Normal/14_August_2008 14] [http://2008.igem.org/Beijing_Normal/15_August_2008 15] [http://2008.igem.org/Beijing_Normal/16_August_2008 16] [http://2008.igem.org/Beijing_Normal/17_August_2008 17]
[http://2008.igem.org/Beijing_Normal/18_August_2008 18] [http://2008.igem.org/Beijing_Normal/19_August_2008 19] [http://2008.igem.org/Beijing_Normal/20_August_2008 20] [http://2008.igem.org/Beijing_Normal/21_August_2008 21] [http://2008.igem.org/Beijing_Normal/22_August_2008 22] [http://2008.igem.org/Beijing_Normal/23_August_2008 23] [http://2008.igem.org/Beijing_Normal/24_August_2008 24]
[http://2008.igem.org/Beijing_Normal/25_August_2008 25] [http://2008.igem.org/Beijing_Normal/26_August_2008 26] [http://2008.igem.org/Beijing_Normal/27_August_2008 27] [http://2008.igem.org/Beijing_Normal/28_August_2008 28] [http://2008.igem.org/Beijing_Normal/29_August_2008 29] [http://2008.igem.org/Beijing_Normal/30_August_2008 30] [http://2008.igem.org/Beijing_Normal/31_August_2008 31]
September
MTWTFSS
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/1_September_2008&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/2_September_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/3_September_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/4_September_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/5_September_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/6_September_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/7_September_2008&action=edit 7]
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/8_September_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/9_September_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/10_September_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/11_September_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/12_September_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/13_September_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/14_September_2008&action=edit 14]
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/15_September_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/16_September_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/17_September_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/18_September_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/19_September_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/20_September_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/21_September_2008&action=edit 21]
[http://2008.igem.org/Beijing_Normal/22_September_2008 22] [http://2008.igem.org/Beijing_Normal/23_September_2008 23] [http://2008.igem.org/Beijing_Normal/24_September_2008 24] [http://2008.igem.org/Beijing_Normal/25_September_2008 25] [http://2008.igem.org/Beijing_Normal/26_September_2008 26] [http://2008.igem.org/Beijing_Normal/27_September_2008 27] [http://2008.igem.org/Beijing_Normal/28_September_2008 28]
[http://2008.igem.org/Beijing_Normal/29_September_2008 29] [http://2008.igem.org/Beijing_Normal/30_September_2008 30]
October
MTWTFSS
    [http://2008.igem.org/Beijing_Normal/1_October_2008 1] [http://2008.igem.org/Beijing_Normal/2_October_2008 2] [http://2008.igem.org/Beijing_Normal/3_October_2008 3] [http://2008.igem.org/Beijing_Normal/4_October_2008 4] [http://2008.igem.org/Beijing_Normal/5_October_2008 5]
[http://2008.igem.org/Beijing_Normal/6_October_2008 6] [http://2008.igem.org/Beijing_Normal/7_October_2008 7] [http://2008.igem.org/Beijing_Normal/8_October_2008 8] [http://2008.igem.org/Beijing_Normal/9_October_2008 9] [http://2008.igem.org/Beijing_Normal/10_October_2008 10] [http://2008.igem.org/Beijing_Normal/11_October_2008 11] [http://2008.igem.org/Beijing_Normal/12_October_2008 12]
[http://2008.igem.org/Beijing_Normal/13_October_2008 13] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/14_October_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/15_October_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/16_October_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/17_October_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/18_October_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/19_October_2008&action=edit 19]
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/20_October_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/21_October_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/22_October_2008&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/23_October_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/24_October_2008&action=edit 24] [http://2008.igem.org/Beijing_Normal/25_October_2008 25] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/26_October_2008&action=edit 26]
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/27_October_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/28_October_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/29_October_2008&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/30_October_2008&action=edit 30] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/31_October_2008&action=edit 31]
Retrieved from "http://2008.igem.org/Team:Beijing_Normal/Notebook"