Team:NTU-Singapore/Notebook/9 June 2008

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(Difference between revisions)
(notebook update)
(Monday 9 June)
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*Choon Kit:
*Choon Kit:
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**Amplification of T7 promoter from the Registry(as gel electrophoresis was not successful last week).
+
**Amplification of T7 promoter, 4 empty plasmids ([http://partsregistry.org/wiki/index.php?title=Part:pSB1A3 pSB1A3], [http://partsregistry.org/wiki/index.php?title=Part:pSB1AT3 pSB1AT3], [http://partsregistry.org/wiki/index.php?title=Part:pSB3K3 pSB3K3], [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3]) from the Registry (as gel electrophoresis was not successful last week).
**Gel electrophoresis running for GFP producer, RBS 0032 and RBS 0034.
**Gel electrophoresis running for GFP producer, RBS 0032 and RBS 0034.
*Chin Chong, Darius: (to be updated)
*Chin Chong, Darius: (to be updated)

Revision as of 02:11, 10 June 2008

Monday 9 June

  • Hung,Zhen Fu:
    • Prepare plasmid DNA(in liquid form) of :
      1. GFP producer x 2
      2. RBS 0032 x 2
      3. RBS 0034 x 2
    • Prepare digestion solutions for 6 above plasmids for later gel electrophoresis.
  • Choon Kit:
    • Amplification of T7 promoter, 4 empty plasmids ([http://partsregistry.org/wiki/index.php?title=Part:pSB1A3 pSB1A3], [http://partsregistry.org/wiki/index.php?title=Part:pSB1AT3 pSB1AT3], [http://partsregistry.org/wiki/index.php?title=Part:pSB3K3 pSB3K3], [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3]) from the Registry (as gel electrophoresis was not successful last week).
    • Gel electrophoresis running for GFP producer, RBS 0032 and RBS 0034.
  • Chin Chong, Darius: (to be updated)