Team:Warsaw/Calendar-Main/20 July 2008

From 2008.igem.org

(Difference between revisions)
Line 2: Line 2:
<!-- do not edit above me! -->
<!-- do not edit above me! -->
-
 
+
<html>
-
<p>Results of ligation: 5 colonies grown<br>Each colony cultured overnight in LB + ampicillin medium</p>
+
<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
-
 
+
Paweł</h3>
 +
<p><ol><li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inocluated on previous day</li>
 +
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with NdeI and NotI. Zero positive results obtained - just empty vectors</li>
 +
<li>Another overnight<a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation>ligation</a> of pET15b-OmpA-omega (NdeI/NotI cutted) with protein Z DNA</li>
 +
</ol></p>
 +
</html>
<!-- do not remove this! -->
<!-- do not remove this! -->
{{WarNotebookEnd}}
{{WarNotebookEnd}}

Revision as of 16:48, 2 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

  1. Isolation of plasmids from cultures inocluated on previous day
  2. Control digest of isolated plasmids with NdeI and NotI. Zero positive results obtained - just empty vectors
  3. Another overnightligation of pET15b-OmpA-omega (NdeI/NotI cutted) with protein Z DNA

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/20_July_2008"