Team:Warsaw/Calendar-Main/22 July 2008

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<p><ol><li>Results of ligation: 6 colonies grown.</li>
<p><ol><li>Results of ligation: 6 colonies grown.</li>
<li>Each colony cultured overnight in LB + ampicillin</li></ol></p>
<li>Each colony cultured overnight in LB + ampicillin</li></ol></p>
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<h3>Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA<br>Antoni</h3>
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<li> Colony PCR on colonies from plates with transformations pGeneart+A Primers used:
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>
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<li> Lack confirmed transformant colonies </li>
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Revision as of 20:13, 2 October 2008

Gallery Bricks Notebook Team Project Home


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Cloning of protein A DNA to OmpA constructs
1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_omega).
2. Control digest of isolated plasmids with BamHI and SacI .

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

  1. Results of ligation: 6 colonies grown.
  2. Each colony cultured overnight in LB + ampicillin


Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA
Antoni

  1. Colony PCR on colonies from plates with transformations pGeneart+A Primers used: AP+NotI and AL+SacI
  2. Lack confirmed transformant colonies

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