Team:Warsaw/Calendar-Main/28 July 2008

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Oczyszczanie białek: A-alfa, Z-alfa i Z-omega
 
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Piotr
 
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Zaszczepienie A-alfa, Z-alfa i Z-omega w rosetach na Lb
 

Revision as of 23:25, 2 October 2008

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1. Digest of pACYC177+OmpA_omega_deltaA_alpha (from 25 July) with BamHI and NotI. DNA ends blunting with Klenow fragment (3 hr).
2. Gel elctrophoresis and gel-out of proper band - 4300 bp.
3. Ligation of isolated DNA fragment (1 hr).
4. Transformation of E. coli TOP10 strain with ligation.
5. Transformants plating on LB + kanamycin.




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