Team:Warsaw/Calendar-Main/2 June 2008

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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a>  with <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a>.</li>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  with <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a>.</li>
<li> Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.</li></ol></p>
<li> Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.</li></ol></p>

Revision as of 23:13, 4 October 2008

Gallery Bricks Notebook Team Project Home


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Piotr:

  1. Transformation of E. coli TOP10 with pZC320.
  2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.

PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase
Michał K.:

Primers:
AIDlNrH and T7pXbSal
Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)
Elongation temperature: 55°C
Time: 4 minutes
Number of cycles: 20


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