From 2008.igem.org
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| | <h4>Michał K.:</h4> | | <h4>Michał K.:</h4> |
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| - | <li> Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a> with HindIII and NcoI (1x Tango buffer).</li>
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| - | < li> DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols# DNA_isolation_from_agarose_gel"> isolation of DNA</a> encoding AID from proper band ( 600 bp).</ li> | + | 1. E. coli < a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols# electrotransform"> electroporation</a> with ligation ( pMPM-T5+AID).</ p> |
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| - | <li> Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> with HindIII and NcoI (1x Tango buffer), <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation</a> (CIAP).</li> | + | <p>2. Plating on LB + tetracycline.</p> |
| - | <li> DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> vector from proper band (4250 bp).</li>
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| - | <li>Electrophoresis of samples of isolated DNA.</li>
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| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments. </li>
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| - | <html><div align="center"><img src="http://openwetware.org/images/e/e1/UW_AID.jpg" height="340"/></div></html>
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Revision as of 08:54, 5 October 2008
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Michał K.:
1. E. coli TOP10 electroporation with ligation (pMPM-T5+AID).
2. Plating on LB + tetracycline.
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