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| | <h4>Michał K.:</h4> | | <h4>Michał K.:</h4> |
| - | <p><ol>
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| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - AID for translational fusion. <br>
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| - |
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| - | Primers:
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| - |
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| - |
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| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a>
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| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDpLinB">AIDpLinB</a>
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| - | <br>
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| - | Template DNA: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a>
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| - | <br>
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| - | Annealing temperature: 55 °C<br>
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| - |
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| - | Elongation time: 60 sec<br>
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| - |
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| - | 20 cycles
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| - | </li>
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| - |
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| - | <li> <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA-polymerase for translational fusion; Taq polymerase with supplemented buffer (Łukasz Banasiak helped us with that problematique PCR).<br>
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| - |
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| - | Primers:
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| - |
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| - |
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| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7lLinkB">T7lLinkB</a>
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| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a>
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| - | <br>
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| - |
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| - |
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| - | Template DNA: <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> genomic DNA<br>
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| - |
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| - | Annealing temperature: 62 °C<br>
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| - |
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| - | Elongation time: 4 minutes<br>
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| - |
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| - | 20 cycles<br>
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| - | </li>
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| - |
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| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA-polymerase for transcription fusion.<br>
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| - |
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| - | Primers:
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| - |
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| - |
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| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7lRBSHi">T7lRBSHi</a>
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| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a>
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| - | <br>
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| - |
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| - |
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| - | Template DNA: <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> genomic DNA<br>
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| - |
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| - | Annealing temperature: 62 °C<br>
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| - |
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| - | Elongation time: 4 minutes<br>
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| - |
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| - | 20 cycles
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| - | </li>
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| - | </p>
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| - | </html>
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| | | | |
| | + | <p>1. Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008">16 May</a> PCR's) and DNA <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> from proper bands (600 bp for AID lane and 2700 bp for T7 polymerase lanes).</html> |
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| | <!-- do not remove this! --> | | <!-- do not remove this! --> |
| | {{WarNotebookEnd}} | | {{WarNotebookEnd}} |
"
