Team:UNIPV-Pavia/Protocols/PlasmidExtraction

From 2008.igem.org

(Difference between revisions)
Line 30: Line 30:
<h2>QIAGEN QIAprep Spin Miniprep Kit</h2>
<h2>QIAGEN QIAprep Spin Miniprep Kit</h2>
-
''(estimated time: 1 hour1)''
+
''(estimated time: 1 hour)''
<br>
<br>
<br>
<br>

Revision as of 12:21, 10 June 2008

Home.jpg Home Unipv logo.jpg The Team And.jpg The Project Safety.jpg Biological Safety Dna.png Parts Submitted to the Registry
Laptop.jpg Dry Lab Pipette.jpg Wet Lab Math.gif Modeling Note.jpg Protocols Notebook.gif Activity Notebook


Plasmid extraction

We used QIAGEN Plasmid Mini Kit in the first 2 weeks of our activity to perform miniprep, when we didn't have QIAGEN QIAprep Spin Miniprep Kit at our disposal. Then, we always used QIAGEN QIAprep Spin Miniprep Kit for its higher yield.

QIAGEN Plasmid Mini Kit

(estimated time: 3 hours)

Materials needed:

  • Overnight mini-culture
  • QIAGEN Plasmid Mini Kit



QIAGEN QIAprep Spin Miniprep Kit

(estimated time: 1 hour)

Materials needed:

  • Overnight mini-culture
  • QIAGEN QIAprep Spin- Miniprep Kit