Team:Warsaw/Calendar-Main/27 May 2008

From 2008.igem.org

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<html><h3>Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7</h3>
<html><h3>Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7</h3>
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<h4>Michał K.:</h4>
<h4>Michał K.:</h4>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5 with transcription fusion.</li>
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<li> Inoculation some separate transformant colonies for transcription fusion (liquid LB + tetracycline).</li>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5 with translation fusion.</li>
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with transcription fusion with EcoRI and XhoI (2x Tango buffer).</li>
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translation fusion with BamHI (BamHI buffer).</li>
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translation fusion with EcoRI and XbaI (2x Tango buffer).</li>
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<li> Choice of proper clones and preparation for sequencing.</li>
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<h3>Here we found the clone with translational fusion on pMPM. These are different isolations in each lane. We have been working with the AT6 ever since. <br>
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Digest was conducted with BamHI</h3>
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<center>https://static.igem.org/mediawiki/2008/6/62/Trawienie_06_05_2008.jpg</center>
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<li> Inoculation some separate transformant colonies for translation fusion (liquid LB + tetracycline). </li>
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</ol></p> </html>

Revision as of 12:41, 5 October 2008

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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.:

  1. Inoculation some separate transformant colonies for transcription fusion (liquid LB + tetracycline).
  2. Inoculation some separate transformant colonies for translation fusion (liquid LB + tetracycline).




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