Team:Warsaw/Calendar-Main/18 July 2008

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<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
Paweł</h3>
Paweł</h3>
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<p><ol><li>Ligation transformed into TOP10 strain and plated on LB+kanamycin</li>
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<p><ol><li>Ligation <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">transformed</a> into <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain and plated on LB+kanamycin</li>
</ol></p>
</ol></p>

Revision as of 16:55, 5 October 2008

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Cloning of protein A DNA to OmpA constructs

  1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega and pACYC177+OmpA_A_alpha. Primers used: AL+SacI and AP+NotI
  2. Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha plate) inoculated to liquid LB with kanamycin.

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

  1. Ligation transformed into TOP10 strain and plated on LB+kanamycin

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