Team:Warsaw/Calendar-Main/2 June 2008

From 2008.igem.org

(Difference between revisions)
Line 4: Line 4:
<html>
<html>
-
<h3>Piotr:</h3>  
+
<h4>Piotr:</h4>  
<p>
<p>
<ol>
<ol>
Line 11: Line 11:
<li> Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.</li></ol></p>
<li> Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.</li></ol></p>
-
 
+
<h4>Michał K.:</h4>
-
<h3><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> for AID transcription fusion with translation fusion AID+T7 RNA-polymerase <br>Michał K.:</h3>
+
<ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> for AID transcription fusion with translation fusion AID+T7 RNA-polymerase <br></li>
-
<p>
+
</ol><p>
Primers: <br>
Primers: <br>

Revision as of 17:41, 5 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Piotr:

  1. Transformation of E. coli TOP10 with pZC320.
  2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.

Michał K.:

  1. PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase

Primers:
AIDlNrH and T7pXbSal
Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)
Elongation temperature: 55°C
Time: 4 minutes
Number of cycles: 20


Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/2_June_2008"