Team:Warsaw/Calendar-Main/3 July 2008

From 2008.igem.org

(Difference between revisions)
Line 11: Line 11:
<li> <A HREF="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_alfa and pET15b. </li>
<li> <A HREF="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_alfa and pET15b. </li>
<li> <A HREF="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_omega and pET15b.</li>
<li> <A HREF="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_omega and pET15b.</li>
-
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> TOP10 strain with ligation products. </li>
+
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htmtop10">TOP10</a> strain with ligation products. </li>
<li> Transformants plating on LB + ampicillin.</li></ol>
<li> Transformants plating on LB + ampicillin.</li></ol>
</p>
</p>

Revision as of 21:40, 5 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 


Preparation of constructs with OmpA protein fusions
Paweł

  1. Clean-up of overnight digest.
  2. Ligation of OmpA_alfa and pET15b.
  3. Ligation of OmpA_omega and pET15b.
  4. Transformation of E. coli TOP10 strain with ligation products.
  5. Transformants plating on LB + ampicillin.