Team:Warsaw/Calendar-Main/4 September 2008

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(New page: {{WarNotebook}} <!-- do not edit above me! --> Oczyszczanie białek: A-alfa, Z-alfa i Z-omega Piotr Zaszczepienie chodowlą nocną 100ml pożywki, wieczorem zaszczepienie tymi 100m...)
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<h3>Purification of proteins: A-alpha, Z-alpha and Z-omega<br>Piotr</h3>
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<p><ol><li>100ml of medium inoculated with overnight culture, then used as an inoculum for 1l culture with presence of Ampicillin, Kanamycin and IPTG (according to <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o>protocol</a>)</li></ol></p>
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<h3>Protein A mutagenesis<br>Paweł</h3>
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<p><ol><li>Results of mutagenesis: 0 colonies on each plate. </li>
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<li>Mutagenesis repeated with modified conditions: fresh stock of dNTPs used and additional MgCl2 added.</li></ol></p>
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Oczyszczanie białek: A-alfa, Z-alfa i Z-omega
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Piotr
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Zaszczepienie chodowlą nocną 100ml pożywki, wieczorem zaszczepienie tymi 100ml pożywki 1l pożywki z Ap, Kn i IPTG (odnośnik do protokołów z oczyszczania)
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Revision as of 22:51, 5 October 2008

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Purification of proteins: A-alpha, Z-alpha and Z-omega
Piotr

  1. 100ml of medium inoculated with overnight culture, then used as an inoculum for 1l culture with presence of Ampicillin, Kanamycin and IPTG (according to protocol)


Protein A mutagenesis
Paweł

  1. Results of mutagenesis: 0 colonies on each plate.
  2. Mutagenesis repeated with modified conditions: fresh stock of dNTPs used and additional MgCl2 added.

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