Team:Warsaw/Calendar-Main/5 September 2008
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PPrzanowski (Talk | contribs) (New page: {{WarNotebook}} <!-- do not edit above me! --> Oczyszczanie białek: A-alfa, Z-alfa i Z-omega Piotr Zwirowanie bakterii z 1l, sonikacja ich (protokół) zwirowanie sonikatu w przypa...) |
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+ | <h3>Purification of proteins: A-alpha, Z-alpha and Z-omega<br>Piotr</h3> | ||
+ | <p><ol><li>Bacteria centrifuged and sonicated (according to <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o>protocol</a>).</li> | ||
+ | <li>Sonicate centrifuged. </li> | ||
+ | <li>In case of Z-alpha and Z-omega: supernatant discarded, pellet resuspended in ddH20, centrifuged and again resuspended in ddH20</li> | ||
+ | <li>In case of A-alpha: pellet discarded, supernatant kept for further purification steps</li></ol></p> | ||
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+ | <h3>Protein A mutagenesis<br>Paweł</h3> | ||
+ | <p><ol><li>Treatment of mutageneses as on 2nd September. </li></ol></p> | ||
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Revision as of 22:58, 5 October 2008
Purification of proteins: A-alpha, Z-alpha and Z-omega
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