Team:Warsaw/Calendar-Main/17 June 2008

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Revision as of 10:51, 6 October 2008


	Attribution analyzer / Interactive list of topics

PCR on OmpA_linker and linker_alpha with OmpaL_N and AlphaP_XB primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57°C).
PCR on OmpA_linker and linker_omega with OmpaL_N and OmegaP_EPB primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57°C).
Gel electrophoresis of PCR products and gel-out of OmpA_alpha and OmpA_omega.
Overnight digest of purified OmpA_alpha and OmpA_omega DNA with NdeI and BamHI.

@@ Line 7: / Line 7: @@
 <ol>
 <li>Repetition of experiment from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/11_June_2008">11/06/2008.</a></li></ol>
+<h3>Preparation of constructs with OmpA protein fusions</h3>
+<h4>Michał K.</h4>
+<p><ol>
+<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on OmpA_linker and linker_alpha  with
+<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">AlphaP_XB</a>
+ primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57&deg;C).</li>
+<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on OmpA_linker and linker_omega with
+<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a>
+ primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57&deg;C).</li>
+<li> Gel electrophoresis of PCR products and
+<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of OmpA_alpha and OmpA_omega. </li>
+<li> Overnight <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of purified OmpA_alpha and OmpA_omega DNA with NdeI and BamHI. </li></ol>
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