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Edinburgh/Week 16
From 2008.igem.org
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* ''cenA'' (M237~238) and ''cex'' (M233~235) biobricks confirmed. Ribosome binding sites are now being added. (CF) | * ''cenA'' (M237~238) and ''cex'' (M233~235) biobricks confirmed. Ribosome binding sites are now being added. (CF) | ||
* ''bglX'' clones (M240, 247) were all wrong. Troubleshooting in progress. (CF) | * ''bglX'' clones (M240, 247) were all wrong. Troubleshooting in progress. (CF) | ||
| - | * | + | * ''ISA1'' (M229) and ''ISA2'' (M231) clones correct but contained messed-up suffixes. Other clones which, by digestion, seem to have intact XbaI and Spe1 sites have been submitted for sequencing. (CF) |
* ''Plac-dxs-lims-appY'' device is ready to test. Hope to do next week if the GC is available. (CF) | * ''Plac-dxs-lims-appY'' device is ready to test. Hope to do next week if the GC is available. (CF) | ||
* Attempts to make ''crt'' construct failed for some unexplained reason - apparently no DNA in the ligations. (CF) | * Attempts to make ''crt'' construct failed for some unexplained reason - apparently no DNA in the ligations. (CF) | ||
Revision as of 19:26, 6 October 2008
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Week 16
- lac promoter added to glgC and glgC16 biobricks, which were then grown overnight in LB and LB+40mM glucose.
- Cells were subsequently resuspended in iodine reagent to test glycogen levels - results are quite promising. See Results section. (CF)
- xylE and lacZ reporter genes added to cstA promoter, then grown overnight in LB and LB+40mM glucose.
- xylE assays performed - results are very promising. See Results section. (CF)
- cenA (M237~238) and cex (M233~235) biobricks confirmed. Ribosome binding sites are now being added. (CF)
- bglX clones (M240, 247) were all wrong. Troubleshooting in progress. (CF)
- ISA1 (M229) and ISA2 (M231) clones correct but contained messed-up suffixes. Other clones which, by digestion, seem to have intact XbaI and Spe1 sites have been submitted for sequencing. (CF)
- Plac-dxs-lims-appY device is ready to test. Hope to do next week if the GC is available. (CF)
- Attempts to make crt construct failed for some unexplained reason - apparently no DNA in the ligations. (CF)
