Team:Warsaw/Calendar-Main/18 June 2008

From 2008.igem.org

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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of purified DNA (pACYC177 with OmpA_alpha and OmpA_omega DNA fragments).</li>
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of purified DNA (pACYC177 with OmpA_alpha and OmpA_omega DNA fragments).</li>
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with ligation products.</li>
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with ligation products.</li>
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<li>Plating transformants on LB+tetracycline.</li></ol></html><br></ol>
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<li>Plating transformants on LB+tetracycline.</li></ol></html><br>
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Revision as of 23:27, 6 October 2008

Gallery Bricks Notebook Team Project Home


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Michał L., Ewa, Marcin:

  1. Counting of blue colonies. Same results as on 12/06/2008.

Michał K.:

  1. Isolation of pACYC177.
  2. Digest of purified pACYC177 with NdeI and BamHI.
  3. Gel electrophoresis and isolation of proper band (3200 bp).
  4. Ligation of purified DNA (pACYC177 with OmpA_alpha and OmpA_omega DNA fragments).
  5. Transformation of E.coli TOP10 with ligation products.
  6. Plating transformants on LB+tetracycline.

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