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Team:Illinois/Antibody Receptor Tyrosine Kinase Fusion Notebook
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| + | == July 1, 2008 == | ||
| + | Made Yeast Media | ||
| + | |||
| + | YPD Medium, per liter: | ||
| + | |||
| + | 10g yeast extract | ||
| + | |||
| + | 20g peptone | ||
| + | |||
| + | 20g dextrose | ||
| + | |||
| + | 20g agar(only for plates) | ||
| + | |||
| + | 1. Dextrose filter sterilized, the rest autoclaved | ||
| + | |||
| + | 2. Weigh nutrients into flask double the volume you want to make, and stir to dissolve | ||
| + | |||
| + | 3. Dextrose added to autoclaved media to equivalent of 20 g/L | ||
| + | |||
| + | 4. Liquid media placed on bench, plate media placed in 65 degree water bath approximately 5 minutes | ||
| + | |||
| + | 5. Poured into plates and allowed to solidify | ||
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Revision as of 01:24, 8 October 2008
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July 1, 2008
Made Yeast Media
YPD Medium, per liter:
10g yeast extract
20g peptone
20g dextrose
20g agar(only for plates)
1. Dextrose filter sterilized, the rest autoclaved
2. Weigh nutrients into flask double the volume you want to make, and stir to dissolve
3. Dextrose added to autoclaved media to equivalent of 20 g/L
4. Liquid media placed on bench, plate media placed in 65 degree water bath approximately 5 minutes
5. Poured into plates and allowed to solidify
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